Abstract

A B S T R A C TSeed multiplication in potato cultivation is critical for its expansion, and in vitro seedling multiplication is chosen to ensure high-quality production and an adequate quantity. In-depth knowledge of the culture medium is essential for success in this technique. Different concentrations of sucrose in the Murashige and Skoog medium (MS) were evaluated for in vitro multiplication of three varieties of Reno potatoes. A 3x4 completely randomized design was used, including three varieties (Laldas, Lulimile, and Kholophete) and four sucrose concentrations (25, 30, 35, and 40 g/l). After 30 days of micropropagation, data were collected for fresh mass, leaf number, root number, and stem length. Normality tests (Shapiro-Wilk), ANOVA, and Tukey's test (α=0.05) were performed for mean comparisons. The maximum dose of technical efficiency (DMET) was calculated for each variable. Interaction between variety and concentration was observed for the variables root number and stem length. It is concluded that 35.3 g/l and 31.96 g/l of sucrose were the best sources and sugar concentrations for fresh mass and leaf number, respectively, for all varieties. The Lulimile variety responded better to the maximum technical efficiency dose (33.97 g/l) for the number of roots.

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